Part:BBa_K2707008
var1
Usage and Biology
var1 encode ribosomal protein in mitochondrion. Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane . uS3m is essential for mitochondrial protein synthesis and required for the maturation of small ribosomal subunits .
Characterization
It's too hard to characterize this part in mitochondrial due to the difficulty of transfering DNA into mitochondrial.However, we try our best to characterize this part. Mitochondrial genome's GC content is very low. When we construct this genome, We found that DNA fragments with low GC content replicated in cells and cause cytotoxicity. So We constructed this part on the pSB1C3 backbone and examined the growth curve of this part in E.coli replication. Used to detect whether a large amount of replication of the gene is toxic to cells.
Figure 1. Growth curve of E.coli which was transfered into cob gene.
Figure 2. The most right lane is marker whose length has been showed in the figure. The lane labeled “1” and “2” is segment of gene named VAR1 ,which is 1197bp as showing in the figure. .
Conclusion
We can see two curve is similar. we can infer that this gene is not toxic to cells when it replication in a high copy mode.
Others
The chassis is DH5alpha.
Blank is DH5alpha which was transfered into pSB1C3 circle plasmid
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |